Air bubbles can create such flow anomalies. 5. 3. Set flow to 0ml/min f. Purging air from pump system: Change solvent line to a bottle containing 100% isopropanol. I usually let the column drip into a beaker while washing it. The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. 1. If using MassLynx or Empower software, refer to the PDFs entitled Priming the 1525 Binary HPLC Pump Using MassLynx or Priming the 1525 Binary HPLC Pump Using Empower. Inspect the springs. The system pressure and PreColumn pressure also fluctuates between 0. Press the Inject button in Empower to wait for injection. Seal wash holes Allow you to flush and clean the plunger seals. 3. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). As well as excellent accuracy, it has very small delay volumes of ≤ 350 μL. Buffer precipitation in LPG pumps. 6 mm ID columns over the flow rate range up to 5 mL/min. 6:51. . 1. Prime the pump with degassed solvent. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales. (Prime each pump head separately. 05. Treatment 2: Open the compression fitting at the top of the outlet check valve with a slow pump flow until solvent leaks around the fitting. operating pressure (9,99 ml/min max. Blockages can also be in front of the degasser, meaning the solvent inlet filters in the solvent bottle. This guide describes the basic operation and troubleshooting for the ExionLCTM AD system . Thus, the gradient delay volume of an HPLC system is the volume that the pump must deliver until a change in solvent composition reaches the column inlet. Run through the prime purge valve first (10 mls), then. 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide. b. It also enables quaternary solvent delivery at. Among the various technologies developed for chromatography,. The pump (s) should be run (column disconnected and bypassed) run with a solution of pure HPLC grade water to try and rinse the material off. Dear Huq, check out the whole tubing starting from Pump to the Column inlet for any air trapped, purge the pump for few minutes. e. . 6 Withdraw ~10ml of water and close the purge valve. The 1290 Infinity II Flexible Pump is a quaternary pump that provides UHPLC gradient formation and solvent blending from up to four solvents. The EVAP system is designed to prevent fuel vapors from escaping into the atmosphere by capturing and temporarily storing them in a. If problem persists, flush system with 100% methanol or isopropanol. Flexible Pump The Agilent 1290 Infinity II Flexible Pump improves your efficiency by combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump. In such case clogging of the column is most likely happening due to: additive (i. 결과 및 정리. Prepare the Pump • Use fresh or different mobile phase (as required). flow rate) One of the most important performance measures for HPLC is retention time reproducibility. If mobile-phase outgassing occurs within the LC system, the most common problem areas are the pumps and detector. purge를 실행하기 전에 꼭 DRAIN 밸브를 열어주고 실행하며, purge가 끝난 후 밸브를 다시 닫아줍니다. Open the "drain" on LC-20AD. By the end of this video you will learn how to safely and quickly swap that suck. To start purging, press the "Purge" bottom on both LC-20AD and SIL-20AC. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting solvent of your application at a reasonable flow rate to rinse out any other solvent; Install the appropriate HPLC column for your application; Close all doors of the. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. Priming the 1525 Binary HPLC Pump Using Empower When to Prime Priming and purging is necessary to ensure proper pump operation when: • You start the pump for the first time • You change eluent • The system has been idle for some time Parts Required None Tools/Materials Required • Priming syringe (in Startup Kit; WAT010337) Procedure 1. 1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision < 0. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. Run at high flow: Next, Dolan advises to run the pump for several minutes at high flow (e. • Syringe, loop & injector plunger seal. 8 Click pump on the flow diagram and change the flow path into A2 and B2. A flow rate of 5mL/min is commonly used for standard HPLC systems. The Waters 1500 Series HPLC Pump Installation and Maintenance Guide has five chapters and four appendixes. So for example, if you normally run at 100 bar and the pressure drops to 5 bar, then the pump will shut down. A damping unit is connected between the two plunger chambers. 5 ml/min for 4 min, I only collected 1 ml of liquid. The good news is that there is a simple solution: open the purge valve, turn the flow rate to five mLs a minute, let it sit there for 10 to. Perform 2 purge cycles. Flush the pump with 100% filtered, distilled water for at least 3 minutes. GasesUltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. Changing the Purge Valve Frit/Purge Valve for Binary Pumps Headquarters | Other sites. 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 ml/min. L/mni for 5mni . 00 ml/min, turn the pump back ON and close the prime purge valve to allow the liquid to flow through solvent lines, through the degasser channel, through the pump. |The 1290 Infinity II Bio Flexible Pump. From my little experience i learnt that it may be due to air trapped in. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. Purge valve: Reciprocating piston pumps do not work if there is air in the lines, which can be the case after changing a solvent reservoir, or leaving the pump off. Pump systems: HPLC pumps can be single, binary, or ternary, meaning that the can make use of one, two, or more different solvent reservoirs. Purge HPLC pumps with buffer made in step 1. With the Arc HPLC, you can easily replicate and improve the performance of. Blockage of. 4. Purification Solvent Management. 8:42. See your pump modules user manual for detailed instructions on re-assembly. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. Flush the column with 10/90 water/ACN for 5 min at 1. Flush the pump with 100% filtered, distilled water for at least 3 minutes. Now vapors which have been taken in by the pump may be compressed. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Algae Growth in HPLC Systems 53 5 Optimizing Performance 55. Thermo Scientific Dionex UltiMate 3000 Series Nano/Cap System NCS-3500RS Nano/Cap Pump NCP-3200RS Operating Instructions (Original Operating Instructions)This instalment is the first of a series of four white papers on high performance liquid chromatography (HPLC) modules (pump, autosampler, UV detector, and chromatography data system) to be published in 2019. One option is to apply vacuum during filtration of mobile phase through 0. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications,Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. Flush the system. Algae Growth in HPLC Systems 81 Prevent Blocking of Solvent Filters 82 Normal Phase Applications 83 4 Optimizing Performance 85 Using the Degasser 86. During step 1, check that the solvent moves into the solvent line correctly and it goes out the Purge Valve. Some bubbles may be generated from the suction filter head dipping in the mobile phase. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. The binary Semipreparative High-Pressure Gradient P680 Pump is ideal for small-scale purification applications. 1:59. methanol and purge the autosampler with methanol for at least 20 minutes. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. 6 Calibration Procedure of HPLC: 6. This will redirect the solvent flow to the waste exit of the valve instead of passing through the. Industry-leading flow and gradient precision. Leak at fittings, purge valve, active inlet If there are still signs of a. Almost all descriptions in the manual apply to all pump models in the LPG-3000 pump series and cover both the standard (stainless steel) and biocompatible pumps. Dolan advises that this will clear. Irreproducible gradient. • Use less-vigorous purging • Prepare fresh mobile phase • Check pump. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . Priming and Purging the 1525 Binary HPLC Pump. Filter all samples and standards 3. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’. It can mainly divided by two types 1. Achieve binary-like performance in your HPLC instrument. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. Maintenance Kits for UltiMate™ 3000 Pumps. • Purge each channe lwtih 25–3m. Weigh the sample into a syringe. Rough pumps stay on so the system remains under vacuum. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. The dual piston design has minimal pulsation for stable baselines. [][]Clean the solvent diffuser/filter once in a week. e. - after changing solvents. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Page II Operating Instructions. Hello, I have dual pump RSLC dionex 3000. ), prime system if necessary. If problem. • Future-proof flow-pressure footprint provides highest application flexibility. 6. The workstation pumps will run at a default flow rate of 10 ml/min and the indicator light will flash green. 7 Repeat steps 4-6 with the next 3 valves. When autosampler inject, HPLC pressure pump falls down and rise again. Typically a 0. The Logi-CHROM pump is compact yet powerful featuring a high quality HPLC pumping system. Then use your nomal mobil phase. g. Once the system is purged, set the flow rate at 0. With Arc HPLC System, you can easily replicate and improve the performance of your existing LC methods without compromising data quality eliminating the burdens of older, less efficient LC systems. The impact of this type of clogging will be gradual, not sudden. A purge valve with The pump assembly includes a pump head with a passive inlet valve and an outlet valve. The manual is in English and can be downloaded as a PDF file. Use only HPLC-grade solvents and water filtered through 0. 3. Open the black purge valve and press the “Purge” button. 11 Click everything on. High concentrations of mobile phase buffer in your system (e. Fig 2. 6:51. It is Liquid Chromatography. From my little experience i learnt that it may be due to air trapped in. the user. The 1525 and 1525 with EF heads are integrated, high-pressure, binary HPLC pumps that feature on-board pulse dampening and efficient mixing. Waters First Quarter 2023 Highlights. If tiny air bubbles are observed in the lines continuously, it’s. Make sure that you know which solvent is linked to which pump. 4. If the column was used with buffered. 1. Initial System inspection It is always worthwhile to spend a few minutes before starting analysis on assessing the system suitability for the analysis task in hand. (Prime each pump head separately. Overlay of 6 pump proportioning chromatograms in 10% gradient. PROCEDURE: Flush the system using one of the cleaning mixtures as described in the cleaning. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. Some pumps will require to manually open the purge valve and in others it is done via the software. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. HPLC is an abbreviation for High Performance Liquid Chromatography. pump program to 90/10 water/ACN to flush the column for 15 min at 1. Always do this one channel at a time (e. 02 to 0. From my little experience i learnt that it may be due to air trapped in. You may use the. Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. After the pump, the mobile phase encounters the autosampler. 1-15 of 17. It also includes relevant guidance for practical handling and mainte-(1:27min) Exchanging the Purge Valve Frit or the Purge Valve (3. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. With regular pump usage, this is recommended every six months. 322 HPLC PumP | uSER’S GuIDE 13 De SC r I pt ION pUMp heaDS Two interchangeable pump head types are available:Passivation is a chemical treatment of stainless steel and purging is a cleaning process. Disconnect your column first and fill your mobile phase bottle with IPA (isopropyl alcohol) start your pump. *Run to run RT variation of ±0. 18: Ready Check Results window appears after the flow meter purge function is If the conclusions from the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX, and performing the Pump Leak Rate Test, points to a defective or clogged AIV, you may try the following procedure: How to Sonicate the Inlet and Outlet Valves of Agilent 1100, 1200 and 1260 Series Pumps. TP01408 RUN STOP EDIT ENTER MENU Waters 515 HPLC Pump Draw-off valve Seal wash hole (not visible) Pump head assembly High. If the pressure does not rise, the purge valve needs to be replaced as it was the cause of high pressure. 2. Air bubbles can create such flow anomalies. ), prime system if necessary. ) If system has check valve, loosen valve to allow air to escape. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Popular answers (1) There can be several reasons for the pressure drops you observe: air could still be present in the system after your recent repair (i. 2. 물론 이건 내 생각이다! 여튼 purge. If the preceding step does not solve the issue, remove the purge valve and start the pump. Certificates. Preparing the Binary Pump 51 Algae Growth in HPLC Systems 52 Setting up the Pump with the Instrument Control Interface 54 Priming the Pump 64 Solvent Information 66. Purging has only helped to reduce. A pump influence is indicated by baseline fluctuations synchronous with the pump cycle. Replacing the Plunger Seal and Plunger. Wash out the blockage. Not Understanding Retention Time Shifts. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. 5. 6 Purging the Pump. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. Chapter/Appendix Description Chapter 1, Overview of the Waters 1500 Series HPLC Pump Describes the. Loss of Power. Hello Kancheri this is the purge valve for Agilent 1260 G1312-60071 | Agilent You can check in the manual at page 154 on HOW to replace purge valve with this. First, disconnect connection (1) in Figure 1 and pump the solvent (to check the backpressure tube 4)). And, remember, if you have a dual-piston pump, you have two piston seals to replace. Cause a:Air bubble in pump head. Page 36 6 Observe the pressure. Liquid Chromatography. This small piece is meant to protect the rest of the system from particulates, and it needs to be replaced regularly. Kata kunci / keywords: HPLC/HPLC instrument, metode analisis/analysis method, metode isolasi/ isolation method, bahan kimia alam/ natural chemical product. Unit conversion calculator for converting commonly used unitsSJ: There are two diffusion paths involved in inline degassing (ignoring vacuum side diffusion). • Syringe, loop & injector plunger seal. a Right-click the pump icon. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. Piston Housing of the Pump Head Assembly: The red arrows show the springs pushing back the pistons. Basic HPLC System Components. Between samples when you are working with new compounds. Figure 7: Pump tab. [][]Fill up & attach Labels of the solvent reservoirs properly. 7. Preparation steps: Flush the pump head with an appropriate solvent using the purge function. First, dissolved gasses in the mobile phase must diffuse to the surface of the membrane or film. During this step, switch the injection and the column selection valves betweenCheck if the spring in the pump head piston housing is broken . Press “Menu” till the purge appears on the screen. 1. Remove sample vials from the auto-sampler tray g. Rebuilding the Reference Valve. g. Priming and Purging the 1525 Binary HPLC Pump. DO NOT PUMP ANY FLUSHING SOLVENT THROUGH THE SEAL WASH LINE, OR INJECTION NEEDLE LINES. You may need to remove and sonicate it. ANSWER: The purge solvent is the solvent being pushed from the pump. Request a Certificate. purge is complete, enter a flow of 1 mL/min and 100% B. Seal could have fluoropolymer O-ring energizer instead (black O-ring). Therefore, the term "the Purge pump and attempt to clean check valves. HPLC maintenance tutorial on how to remove the pump head on an 1100/1200/1260 Agilent HPLC. For more information, visit tutorial demonstrates how to purge the system with Bio-Rad's new NGC liquid chromatography. † Superior quaternary mixing and a delay volume of 350 μL enable shortest run times, highest precision, and accuracy. If problem persists, flush system with 100% methanol or isopropanol. max) right away, reduce the flowrate before checking. don't forget checking the purge valves. Open prime/purge valve (in the case of Chrom Tech pumps, you turn the knob counterclockwise one to two turns. 0Mb)Page 35: Purging The Pump The pump should never be used for priming/purging empty tubings (never let the pump N O T E run dry). 2 Wellplate Autosampler Purge Kit Features Features The new Wellplate Autosampler Purge Kit offers the following features: • Fits for Agilent 1100 Series Wellplate Autosampler (G1367A and G1368A (thermostatted)). Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies coursesRemedy. ( refer to Pump Operating Manual ). *Run to run RT variation of ±0. 1ml/min2 g. On – line vacuum degassing is available on most commercial available systems. If problem persists, flush system with 100% methanol or isopropanol. Maintaining the Purge Valve 139 Maintaining the Pump Head Assembly 140 Procedure 1: Pump Head with old Plunger Housing 141 Stage 1: Removing the Pump Head Assembly 141. It provides HPLC systems with an automatic, continuous method of removing dissolved gases from mobile phases. Vent. 1–1% solution by volume is sufficient. 3601-EN Revision 5. 01. Open the purge valve by turning the knob on the front of the pump to the left until you feel some resistance. From my little experience i learnt that it may be due to air trapped in. Draw syringe back to prime. Use only HPLC-grade solvents and water filtered through 0. 2 Content 4 2. SPE, LLE) on. 1:55. 6 x 300 mm, 3 µm, 150 Å). 8. Purge Solvents a. 400 bar max. Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this manual. 2. Pump a 20% nitric acid/water solution through the pump for 3 minutes. Then wash them out with water and re-assemble them on the pump head. Set the pump to 100% 'A' at 1. In fire and explosion protection engineering, an inert (ie, non-flammable) purge gas (like nitrogen, helium, argon, etc) is introduced into an enclosed system (eg, a container or process vessel) to prevent the formation of a fuming. The manual covers installation, operation,. Waters Second Quarter 2023 Highlights. off. Basic HPLC System Components. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. keep the system at this flow rate for about 10 minutes to equilibrate the system without the Column. Replace the pump head if the wetted surface is damaged, resulting in internal leaks compromising pump performance or if the pressure sensor is faulty. For stainless steel flow paths, proceed to Step 6; For PEEK flow paths, the cleaning procedure is completed. Purge the pumps each time fresh eluent or water is prepared. For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ Dionex™ Ultimate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. First, initially raise the helium flow_rate (adjusting it via pressure to 0. Currently loaded videos. Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low. Agilent University. Purging the solvent pumps 1. The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations: Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons. Load a Method a. ) If system has check valve, loosen valve to allow air to escape. Replace piston seals. 1 Purpose 4 1. Disconnect tubing at guard column (if present) or analytical column inlet. Varian Modular Analytical HPLC Systems Figure 1. HPLC System causes of increased pressure can include obstructions in the flow path, from the pump outlet to the mobile phase waste tube after the detector outlet. With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. In a low-pressure gradient (LPG) pump type, a proportioning valve is used to aspirate the desired solvent mix. How do i manually prime/purge it? Because there are some air bubbles, and if they get inside the needle, its gonna be bad news bears ( i think). . This video shows the operation a HPLC purge valve. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting. 22μm porosity membrane filter. Click the [Auto Purge] button to perform auto purge. Request Information. Feeding the degasser with an inferior mobile phase will create inferior results. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. This video demonstrates the proper procedure to manually prime the pump on a Shimadzu LC 40 instrument. Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump. Relevant functions marked for purging, flow settings ect. The new 1290 Infinity II LC power range has a high instrument efficiency, allowing you to run any HPLC and UHPLC method. Search by lot number or partial lot number. Treatment 1: Open a purge valve. From the data analysis program vent the system. If problem still persists, contact system manufacturer. Purge pump at high flow rate (e. Replacing the Plunger Seal and Plunger. This can be caused by reduced fuel and airflow in the system, resulting in misfires and power loss. Left pump connected to Vector PC post column derivatization. You. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. The sensor signals the control board when the vacuum is below a preset level. If a purge valve is not functioning correctly, it can lead to the vehicle losing power or stalling unexpectedly. I am working with HPLC Shimadzu 20AD fitted with LC20AD. g. • Purge each channel for at least 10 min at 5 mL/min. Check the solvent line filters for permeability. If it does not, check the state of the Solvent Filters and replace them. 1 A) Calibration of Pump:-. Flushing and purging all solvent lines and fluidics is the first step that should be done if unstable system pressure. 1 Introduction Purpose of the User Manual The User Manual provides you with instructions and information to run the ÄKTA™ avant system. Loaded 0%. The manual covers the overview, installation, operation, and optimization of the pump, as well as troubleshooting and maintenance tips. If you cannot see a leak at the bottom of the pump head when you try to purge, then most likely a leak is not the cause of the issue. Rugged and dependable, Arc HPLC System delivers the ideal balance of performance and value for right-the-first-time analysis. Almost all descriptions in the manual apply to all pump models in the LPG-3000 pump series and cover both the standard (stainless steel) and biocompatible pumps. Treatment 1: Open a purge valve. For modular autosamplers purge from front panel. Perform flow rate check. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then to VERIFY that each channel delivers the reported amount of fluid to the column (measure it). between the degasser and the column. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersLC-40D XR Specifications. Press the purge buttons again to. 88 Service Handbook for 1050 Series of HPLC Modules - 11/2001 Pumps: General Information Specifications Specifications Table 2 Specifications of 1050 PumpsHi,when I do wet prime/dry prime in hplc ? Thank you. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. ) If system has check valve, loosen valve to allow air to escape. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. A key element of its user-friendly functionality is the accessibility of the. g. . It can mainly divided by two types 1. Remove and disassemble the pump head. categories. c. 1260 Infinity Binary Pump User Manual 11 Introduction to the Binary Pump 1 Instrument and OperationThe pump assembly includes a pump head with a passive inlet valve and an outlet valve. HPLC pumps are designed to pump liquid, not air. 1:55. Purge pump at high flow rate (e. 2 Low Pressure No/Low Pressure Reading Question Yes No 1. The 1260 Infinity II Flexible Pump combines high performance and simplified operation with highest flexibility in automated gradient formation and solvent blending. Fig. Note: Follow the instructions in the Ready Check Results window and close both purge screws. Figure 6: Pump head with purge valve and outlet to autosampler. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. Overlay of 6 pump proportioning chromatograms in 10% gradient. Consumables likely need to be replaced on aqueous pump. Flow Connections of the Binary Pump with Solvent Selection Valve 24 Flow Connections of the Binary Pump without Solvent Selection Valve 27 Priming and Purging the System 30 2 Optimizing Performance How to optimize the binary pump to achieve best chromato-graphic results 33 Hints for Successful Use of the Binary Pump 34 Solvent. HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. 2. Replace PTFE frit in the purge valve. An optional seal wash function is available for applications using Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact Solving Problems That Matter - Batteries. This pump enables quaternary solvent delivery at pressures up to 1300 bar and flow rates up to 5 mL/min. 3. The user manual for the Agilent G1311A Quaternary Pump, a high-performance liquid chromatography (HPLC) module that provides reliable gradient formation and precise flow rates. The stationary phase is a granular material with very small porous particles in a separation column. Moreover, for system protection, the pump drive is switched off when reaching a pre-defined pressure limit.